The company - one of the largest ESET channel partners in Australia - has also achieved platinum partner status.
The Pro1 acquisition was aimed at diversifying from Microbe's traditional consumer market into the business space.
"ESET's technology was the best solution in the market. We see huge potential for ESET in Australia, both for our resellers and for their customers.
"We will continue to provide great incentives and systems to bring on new resellers during 2015."
Microbe is aiming for double-digit market share growth in Australia.
ESET Software Australia managing director Florin Vasile said "Microbe is a very well-oiled machine, and the team has a lot of experience in selling antivirus solutions. We fully expect this arrangement to strengthen our position and provide even greater results in the coming year."