The trial was carried out at night on 18 November and footage will be broadcast on the Channel 7 news at 6pm AEST.
Hands-free driving is not allowed on roads in Victoria and hence the trial could only be run at a time when the tunnels were closed.
A statement from EastLink said the trial was carried out to help motorists "gain a better understanding of lane keep assist and other driver assistance functions".
“EastLink wants to help motorists gain a better understanding of the latest driver assistance functions, and how they will evolve to make self-driving cars possible in the future.
“With driver assistance functions such as lane keep assist expected to improve road safety significantly, we hope that the demonstration encourages motorists to consider the availability of these new vehicle capabilities when choosing their next car."
EastLink said it selected the Honda Civic VTi-LX for the demonstration after the car was put through trials of the latest automated vehicle technologies undertaken this year by EastLink in partnership with VicRoads, the Australian Road Research Board, La Trobe University and RACV.
The Honda Civic VTi-LX at a toll point on the freeway.
Spencer-Roy said: "The Honda Civic steered itself using lane keep assist mode along EastLink and through the EastLink tunnels at speeds up to 80kmph, while the driver was not holding the steering wheel.
“The Honda Civic lane keep assist function was not affected by changing light conditions during the demonstration, such as the transitions into and out of each tunnel portal.
“The demonstration showed that driver assistance functions in cars are rapidly increasing in quality and availability, which is paving the way for motorists to experience hands-free driving on freeways in the coming years (subject to legislative changes)."
Photos: courtesy EastLink